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Hansatech Fluorometer Interface Settings

Goals

Documents multiple examples of settings for Hansatech fluorometer. Settings are dependent on your project and goals.

Mid-day (oleiodes project)

  1. Actinic light, equivalent to ambient light:

    1. Set value between 0 and 50 (steps from 0 to 3000 micromoles m-2s-1)🡪probably keep at 50, check readout
    2. Need 10 sec of actinic light for measurement

  2. Pulse: saturating light to leaf:

    1. Check peak fluorescence by pulse from 0 to 100
    2. Choose the saturating pulse, without torturing plant with high light (at beginning of plateau value)

  3. Width of pulse: length of pulse

    1. .7 is what Jose uses, check that additional pulse exposure doesn’t change fluorescence adjust to maximize

Yield (oleoides)

In general,there is an initial high yield light exposure, then 10 sec of actinic light, then another saturating pulse

  1. Gain: 50 (check glossary)

  2. Mod: 2 (modulation, check manual if need to adjust)

  3. Log: 1 (1= log data, 0=no logging)

  4. FVFM: ##,##,##

    1. 2.5 duration of element
    2. 60 intensity of saturating pulse (adjust as needed)
    3. .7 width

  5. ACT: 43 actinic

  6. WAIT: 10 (seconds of waiting)

  7. ePS2:

    1. 2.5 duration of element
    2. 60 pulse
    3. .7 width

  8. Beep 1=yes beep

Dark-adapted (ACE)

  1. Gain: 50

  2. Mod: 2

  3. Log: 1

  4. FVFM: ##,##,##

    1. 2.5 duration of element
    2. 60 intensity of saturating pulse
    3. .7 width

  5. Beep: 0.5

Dark-adapted (FAB 2)

Note: Default values for Fv/Fm

  1. Gain: 50

  2. Mod: 2

  3. Log: 1

  4. FVFM: ##,##,##

    1. 2.5 duration of element
    2. 100 intensity of saturating pulse
    3. .7 width

Light-adapted (ACE)

  1. Gain: 50

  2. Mod: 2

  3. Log: 1

  4. ACT: 30 actinic

  5. WAIT: 10

  6. ePS2:

    1. 2.5 duration of element
    2. 50 pulse
    3. .7 width

  7. Beep: 0.5

Light-adapted (FAB 2)

Note: Actinic light set to ~1040 PAR

  1. Gain: 50 (check glossary)

  2. Mod: 2

  3. Log: 1

  4. ACT: 44 actinic

  5. WAIT: 10

  6. ePS2:

    1. 2.5 duration of element
    2. 50 pulse
    3. .7 width
Last update: October 20, 2022